Journal: Oncology Letters

Article Title: Inhibition of Hes1 enhances lapatinib sensitivity in gastric cancer sphere-forming cells

doi: 10.3892/ol.2017.6683

Figure Lengend Snippet: Infection with Hes1-siRNA lentivirus results in impaired EMT and decreased expression of chemoresistance-associated proteins in sphere-forming MKN45 cells. (A) EMT was impaired in sphere-forming cells infected with Hes1-siRNA lentivirus, as demonstrated by a significant decrease in the expression levels of the EMT-associated proteins Snail, N-cadherin and vimentin, and an increase in expression level of E-cadherin and ZO1 compared with those in the control-siRNA group. (B) The expression levels of the chemoresistance-associated proteins MDR1, ABCG1, ABCG2 and RAD51 were significantly downregulated in sphere-forming cells infected with Hes1-siRNA lentivirus compared with those in the control-siRNA group. Protein expression levels were normalized to GAPDH. Data are presented as the mean ± standard deviation (n=5). *P<0.05. Notch1, neurogenic locus notch homolog protein 1; Hes1, transcription factor Hes1; siRNA, small interfering RNA; Snail, zinc finger protein SNAI1; MDR1, multidrug resistance protein 1; ABCG, ATP-binding cassette sub-family G member; RAD51, DNA repair protein RAD51 homolog 1; ZO1, tight junction protein ZO-1; EMT, epithelial-mesenchymal transition.

Article Snippet: Antibodies directed against GAPDH (cat. no. 560005) and zinc finger protein SNAI1 (Snail, cat. no. 550589) were purchased from BD Biosciences (Franklin Lakes, NJ, USA).

Techniques: Infection, Expressing, Standard Deviation, Small Interfering RNA, Binding Assay